The prevalence of cotton leaf curl disease (CLCuD) has significantly hampered chili (Capsicum spp.) production, presenting a formidable challenge in Pakistan.During a chili field survey in 2018, distinct symptoms, including stunted growth, yellowing, and severe leaf curling, were observed on several plants.Subsequently, a comprehensive sampling effort was undertaken, collecting a total of 39 symptomatic samples from Dog Supplements diverse locations across Multan, Punjab.
The DNA extraction from these samples was conducted at the plant virology laboratory at Bahauddin Zakariya University, Multan, marking a crucial step in the investigation of this debilitating disease and its impact on chili production in the Ninja Air Fryer region.Molecular analysis with PCR using Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33 primers confirmed begomovirus infection in chili plants.Positive amplification demonstrated a 71.79% infection rate, with 579 bp, 1.
4 kb, and 481 bp amplicons for Av/Ac Core, Beta 01/02, and CLCuMuBF11/R33, respectively.Sequencing identified cotton leaf curl Multan beta-satellite (MT668934) infecting the chili plant.Effectively managing these begomoviruses is crucial to curbing their multiplication and protecting vital crops like chili.Addressing the distributions of beta-satellites in agricultural fields, particularly chili, is imperative to prevent further viral spread.